In recent years electrospray has become the most popular ionization technique for the analysis of biomolecules. In the present study, we have compared the electrospray and continuous-flow fast-atom bombardment methods of sample introduction and ionization for the analysis of sulphated and sulphonated lipids by tandem mass spectroscopy. The tandem mass spectra obtained when using the two ionization techniques were similar; however, small differences were evident. These differences indicate that the precursor ions generated by fast-atom bombardment and electrospray have different internal energies. The two ionization techniques give similar sensitivities for the recording of collision-induced dissociation spectra. Complete structural information can be obtained from 5 to 50 ng of lipid.

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