The aim of this study was to investigate the epidemiology and transmission of Taenia hydatigena in sheep and dogs from Sardinia and the economic estimation of losses due to this metacestodosis in lambs. A total of 7781 Sarda breed lambs were examined at abattoirs for the detection of Cysticercus tenuicollis or necrotic-haemorrhagic tracks of their migration. Morphological and molecular identification of parasites was carried out. Individual faecal samples from 300 dogs were examined for copromicroscopic investigations and coproELISA assay. An overall prevalence of 14.6% for T. hydatigena cysticercosis was found in the examined lambs. In total, 10,807 parasitary tracks were found, with an abundance of 1.39 and an average intensity of 9.52. The molecular analysis of the isolates showed an overall pairwise nucleotide divergence for the CO1 and ND1 was of 0-3.1 and 0-3.3%, respectively. Low intra- and interspecific variation was recorded for C. tenuicollis isolates used in this study which suggested the absence of differentiation. Microscopic examination of dog faeces showed a total prevalence of 31.3% for endoparasites in the examined samples (94/300). Taeniid eggs were found in 8.3% of the dogs. The results of the monoclonal antibody ATH4 ELISA test showed a prevalence of 11% (33/300) for T. hydatigena coproantigens. The total economic costs related to cysticercosis amounted to almost € 330,000. The prevalence of C. tenuicollis in 14.6% of 30-40-day-old lambs highlights the high parasitic pressure by T. hydatigena in the territory of Sardinia, Italy.

Abstract:This study aimed at determining the field-saturated soil hydraulic conductivity, K fs , of an unmanaged field affected by fire by means of single-ring infiltrometer runs and the use of transient and steady-state data analysis procedures. Sampling and measurements were carried out in 2012 and 2017 in a fire-affected field (burnt site) and in a neighboring non-affected site (control site). The predictive potential of different data analysis procedures (i.e., transient and steady-state) to yield proper K fs estimates was investigated. In particular, the transient WU1 method and the BB, WU2 and OPD methods were compared. The cumulative linearization (CL) method was used to apply the WU1 method. Values of K fs ranging from 0.87 to 4.21 mm·h −1 were obtained, depending on the considered data analysis method. The WU1 method did not yield significantly different K fs estimates between the sampled sites throughout the five-year period, due to the generally poor performance of the CL method, which spoiled the soil hydraulic characterization. In particular, good fits were only obtained in 23% of the cases. The BB, WU2 and the OPD methods, with a characterization based exclusively on a stabilized infiltration process, yielded an appreciably lower variability of the K fs data as compared with the WU1 method. It was concluded that steady-state methods were more appropriate for detecting slight changes of K fs in post-fire soil hydraulic characterizations. Our results showed a certain degree of soil degradation at the burnt site with an immediate reduction of the soil organic matter and a progressive increase of the soil bulk density during the five years following the fire. This general impoverishment resulted in a slight but significant decrease in the field-saturated soil hydraulic conductivity.

A study was carried out on the presence of Anisakis and Hysterothylacium larvae in fish and cephalopods caught in Sardinian waters. A total of 369 specimens of 24 different species of teleosts and 5 species of cephalopods were collected from different fishing areas of Sardinia. Larvae were detected and isolated by both visual inspection and enzymatic digestion. These methods allowed Anisakis type I and type II third-stage larvae and Hysterothylacium third- and fourth-stage larvae to be detected. The prevalence, mean intensity, and mean abundance were calculated. The results obtained showed the highest prevalence of Anisakidae in Zeus faber (100%) and of Anisakis in Micromesistius poutassou (87.5%). The highest prevalence of Anisakis type I larvae was in M. poutassou (81.2%), and that of Anisakis type II larvae was in Todarodes sagittatus (20%). The highest values for prevalence, mean intensity, and mean abundance for Hysterothylacium were found in Z. faber. These prevalences and the mean intensity and abundance were higher than those reported by different authors in other Mediterranean areas. This may be because the enzymatic digestive method used in this research resulted in higher recovery levels. The data suggest that Sardinia may be a high-risk area for zoonotic diseases and that measures such as information campaigns, aimed at both sanitary service personnel and consumers, should be employed to limit the spread of such zoonosis.

The lateral saturated hydraulic conductivity,Ks,l, is the soil property that mostly governs subsurface flow in hillslopes. Determinations ofKs,lat the hillslope scale are expected to yield valuable information for interpreting and modeling hydrological processes since soil heterogeneities are functionally averaged in this case. However, these data are rare since the experiments are quite difficult and costly. In this investigation, that was carried out in Sardinia (Italy), large-scale determinations ofKs,lwere done in two adjacent hillslopes covered by a Mediterranean maquis and grass, respectively, with the following objectives: i) to evaluate the effect of land use change onKs,l, and ii) to compare estimates ofKs,lobtained under natural and artificial rainfall conditions. HigherKs,lvalues were obtained under the maquis than in the grassed soil since the soil macropore network was better connected in the maquis soil. The lateral conductivity increased sharply close to the soil surface. The sharp increase ofKs,lstarted at a larger depth for the maquis soil than the grassed one. TheKs,lvalues estimated during artificial rainfall experiments agreed with those obtained during the natural rainfall periods. For the grassed site, it was possible to detect a stabilization ofKs,lin the upper soil layer, suggesting that flow transport capacity of the soil pore system did not increase indefinitely. This study highlighted the importance of the experimental determination ofKs,lat the hillslope scale for subsurface modeling, and also as a benchmark for developing appropriate sampling methodologies based on near-point estimation ofKs,l.

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