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Random lasers (RLs) rely on obtaining laser emission in disordered systems with gain. The characterization of the RLs are performed by the observations of bandwidth narrowing, increasing in the slope efficiency, time shortening of the upper level of the RL transition, intensity and spectral fluctuations, and even photon statistics transitions when increasing the optical gain. However, the gain clamping, which is a known phenomenon in conventional lasers, i.e. lasers with well-defined cavity, was never investigated in RLs. Here we realize an experimental-theoretical investigation of the gain clamping in RLs, and demonstrate that it can be used as an alternative tool to characterize the transition from spontaneous emission to the RL regime.