saat ini penggunaan depot air minum isi ulang (DAMIU) relatif lebih tinggi, sehingga penggunaan DAMIU harus diperhatikan sanitasi dan higenisitas untuk menunjang agar DAMIU terhindar dari kontaminasi bakteri Coliform, salah satu contoh bakteri Coliform yaitu bakteri Escherichia Coli. bakteri Escherichia coli adalah salah satu penyebab penyakit diare. Diare merupakan penyakit endemis potensial kejadian luar biasa (KLB) yang sering dengan kematian di Indonesia. Pravalensi diare di Indonesia sebanyak 6,8% atau sebanyak 1.017.290 orang dan diketahui bahwa kabupaten Tanah Bumbu peringkat ketiga penyumbang diare terbanyak yaitu sebesar 4,3% atau sebanyak 2006 orang di Kalimantan Selatan, sehingga perlu dilakukan pengujian pada DAMIU di Desa Sungai Danau menggunakan metode MPN.

Self-medication or self-medication is the activity or act of self-medicating with medication without a proper and responsible prescription. Limited activities and activities caused by the COVID-19 pandemic eventually led to limited health care activities. People who have complaints of pain feel more vigilant to check the situation to medical personnel so choose to do the independent treatment. The purpose of this study is to find out the factors that affect the management of traditional drugs and drugs independently in the covid-19 pandemic. This research is descriptive. The sample in this study amounted to 113 people with convenience sampling techniques. The results obtained based on research are known that the management of drugs and traditional medicines independently is influenced by gender, occupation, and distance of the place to buy, based on statistical tests of factors that affect drug management both before the pandemic and after pandemic obtained a value of p 0,000 (<0,05), which means there is a meaningful relationship between drug use both before the pandemic and after the pandemic with gender, work, and the distance from where to buy.

Latar belakang: Daun kapuk randu (Ceiba pentandra (L.) Gaertn) merupakan tanaman yang banyak tumbuh di Kalimantan Tengah yang secara empiris digunakan sebagai penghambat pembentukan serta meluruhkan batu ginjal. Daun kapuk randu diketahui mengandung flavonoid yang mampu menghambat pembentukan dan meluruhkan batu ginjal. Tujuan: Mengetahui kadar flavonoid total ekstrak daun kapuk randu pada fraksi n-heksan, etil asetat dan metanol. Metode: Metode desktiptif observasional dengan melihat hasil dari data kualitatif yaitu dengan uji reaksi warna dan kromatografi lapis tipis (KLT) dan data kuantitatif yaitu dengan spektrofotometri Uv-Vis untuk melihat kadar total flavonoid. Hasil: Hasil analisis kualitatif senyawa flavonoid dengan reaksi warna dan KLT menunjukkan positif mengandung senyawa flavonoid. Hasil analisis kuantitatif dengan spektrofotometri Uv-Vis didapatkan kadar total flavonoid untuk ekstrak etanol 96%, fraksi n-heksan, fraksi etil asetat dan fraksi metanol berturut-turut adalah sebesar 31,663 mg QE / g atau 3,1667%; 5,167 mg QE / g atau 0,5167%; 51,833 mg QE / g atau 5,1833%; dan 7,166 mg QE / g atau 0,7166%. Kadar flavonoid total tertinggi yaitu pada fraksi etil asetat. Kesimpulan: Ekstrak daun kapuk randu dengan tingkatan fraksi mengandung senyawa flavonoid dengan kadar flavonoid total pada ekstrak etanol 96%, fraksi n-heksan, fraksi etil asetat dan fraksi metanol berturut-turut adalah sebesar 31,663 mg QE / g atau 3,1667%; 5,167 mg QE / g atau 0,5167%; 51,833 mg QE / g atau 5,1833%; dan 7,166 mg QE / g atau 0,7166%.

Research of mouthwash formulation and evaluation of kalangkala leaves extract (Litsea angulata) as an oral antiseptic also has been tested in Streptococcus mutans bacteria by variation in the concentration of extract 2%, 2,5%, and 3%, which aims to determine the ideal formula in physical quality and has the highest antibacterial activity based on an inhibition zone of Streptococcus mutans bacteria in mouthwash formula. The methods used include the step of extraction of Kalangkala leaves, preparation of mouthwash with 3 formula F1 (2%), F2 (2,5%), and F3 (3%) followed by an evaluation that includes organoleptic, pH test, viscosity test, and the test of inhibition zone bacteria. The test of inhibition zone bacteria used NA media with diffusion method. Stability was performed on weeks 1, 2, 3, and 4. The result showed variation in the concentration of extract kalangkala leaves in a mouthwash formula affects the diameter of the inhibition zone. But did not have a significant effect on the physical stability properties of the mouthwash formula. Mouthwash formula has the highest antibacterial activity based on the inhibition zone of Streptococcus mutans that is 3% contained in formula F3.

The Gayam tree is widespread in the territory of Indonesia, the Gayam plant (Inocarfus Fegifer Fosb) is also used as a medicine for bloody bowel movements in the community by boiling the stems and drinking boiled water. Besides having medicinal properties, Gayam is also used as food in the community in the Banjarmasin area. The common people certainly do not know the benefits of gayam and its nutritional value. Research on the identification of secondary metabolite compounds in Gayam leaf extract (Inocarpus Fagifer Fosb) using TLC where Gayam Leaves (Inocarpus Fagifer Fosb) was extracted by maceration method, the thick extract obtained was then carried out by testing the identification of secondary metabolites with color reagent and TLC. The results of the identification of the compound metabolite of gayam leaves (Inocarfus Fegifer Fosb) by color reagent and TLC test showed positive test results for alkaloids, flavonoids, terpenoids, saponins.