No abstract

Aflatoxins were extracted from the sample by high-speed blending with aqueous acetone, purified with ammonium sulfate, re-extracted into benzene, evaporated to dryness, and redissolved in chloroform-acetone. The final solution was subjected to descending minicolumn chromatography through successive layers of alumina, silica gel, and Florisil, and the aflatoxin band was visualized by fluorescence. The method requires only 35—40 min, and results are semiquantitative.

Aflatoxins are extracted from the sample by high-speed blending with aqueous acetone, purified with ammonium sulfate, and partitioned into benzene. The benzene extract is purified on a silica gel-alumina column by washing with benzene-acetic acid and ether-hexane, and eluted with methylene chloride-acetone. The eluate is evaporated, redissolved in benzeneacetonitrile, and spotted on a thin layer chromatographic plate for resolution and quantitative determination against standards.