The aim of the article is to study the interaction effect between NAA and BAP concentration on protocorm growth and to know the proper concentration for growth of Dendrobium  spectabile  orchid protocorm. This research method using an experimental method which is conducted in the Laboratory of Agriculture Faculty of Winaya Mukti University, Tanjungsari Subdistrict, Sumedang District. The experiment was conducted from June 2017 until September 2017. The experiment used was a Completely Randomized Design (CRD) with the factorial pattern, consisting of two factors and repeated twice. the first factor was the effect of NAA concentration which consisted of five levels, namely without NAA, 0.5 mg kg-1, 1.0 mg kg-1, 1.5 mg kg-1, and 2.0 mg kg-1.  The second factor is the BAP concentration which consists of five levels, namel without BAP, 1.0 mg kg-1, 2.0 mg kg-1, 3.0 mg kg-1, and 4.0 mg kg-1. Eksplant is protocorm from orchid D. spectabile which is grown on MS medium (Murashig and Skoog) half recipe as base medium accompanied by each treatment for 12 weeks. The experimental results show that there is an interaction between the effect of NAA and BAP concentration on the number of leaves only. Without NAA or 1.5 mg kg-1 NAA concentration with BAP 2.0 mg kg-1 gives more leaves.  Independent of NAA or BAP concentrations did not affect the number of buds, number of roots, root length, fresh and dry weight of plantlets, and also growth ability of plantlets.  BAP concentration only affect plant height, and the highest plantlet height is found without add of BAP in medium culture

Abstract. This research was conducted to determine the effect of concentration of Ethyl Methyl Sulfonate (EMS) to performance of three types of Phalaenopsis Planlets and to know the optimum concentration of EMS on any type of Phalaenopsis orchids. The experiment was conduc-ted at Tissue Culture Laboratory of the Faculty of Agriculture, Winaya Mukti University, Sumedang. It used a completely randomized design (CRD) with factorial pattern, that consisted of two factors and replicated twice.The first factor was the type of Phalaenopsis that resulted form hybridization which consisted of v1 (Phalaenopsis 717 X Phalae-nopsis Fire Bird), v2 (Phalaenopsis Tianong Rose X Sibling), and v3 (Phalaenopsis Luchia Pink X Phalae-nopsis Chain Xen Mammon). The second factor was the concentration of EMS that consisted of e0 (0% EMS/control), e1 (0,05% EMS), e2 (0,10% EMS), e3 (0,15% EMS), e4 (0,20% EMS), and e5 (0,25% EMS). Explant protocorm of three types of Phalae-nopsis soaked in a solution of EMS by each treat-ment for 3 hours, and cultured on MS medium Basal Modified Multiplication Shoot for 10 weeks. The experimental results showed that there are interaction between the three types of Phalae-nopsis result of a cross with a concentration of EMS to variable number of roots. Orchids P. 717 X P. Fire Bird had higher  number of leaves, number of roots, leaf leang, and root length than the other. The result showed that there were interaction between three species of Phalaenopsis orchid from the crossing with EMS concentration on root variables. Orchid P. 717 X P. Fire Bird has the number of leaves, the number of roots, the number of shoots, leaf length and root length better than other types. EMS concentrations independently at all levels of treatment can not increase the number of leaves, the number of roots, fresh weight of planlet, leaf length, and root length.Keywords: Phalaenopsis, the concentration of EMS, in vitro cultureSari. Penelitian ini mempelajari dan mengetahui pengaruh konsentrasi Ethyl Methyl Sulfonate (EMS) terhadap keragaan planlet tiga jenis anggrek Phalaenopsis asal protocorm dan mencari konsentrasi optimum EMS untuk setiap jenis anggrek Phalae-nopsis secara in vitro.Percobaan dilaksanakan di Laboratorium Kultur Jaringan Fakultas Pertanian Universitas Winaya Mukti Sumedang. Eksperimen menggunakan Rancangan Acak Lengkap (RAL) Pola Faktorial, yang terdiri atas dua faktor dan diulang sebanyak dua kali.Faktor pertama adalah jenis anggrek Phalaenopsis hasil persilangan yang terdiri atas tiga jenis, yaitu v1 (Phalaenopsis 717 X Phalaenopsis Fire Bird), v2 (Phalaenopsis Tianong Rose X Sibling), dan v3 (Phalaenopsis Luchia Pink X Phalaenopsis Chain Xen Mammon). Faktor kedua adalah EMS yang terdiri atas enam taraf perlakuan, yaitu e0 (0% EMS/kontrol), e1 (0,05% EMS), e2 (0,10% EMS), e3 (0,15% EMS), e4 (0,20% EMS), dan e5 (0,25% EMS). Eksplan berupa protocorm dari tiga jenis anggrek Phalaenopsis hasil persilangan yang direndam dalam larutan EMS berdasarkan masing-masing perlakuan selama 3 jam, dan dikulturkan pada media MS Modified Multiplication Shoot Basal selama 10 minggu.Hasil penelitian menunjukkan terjadi interaksi antara tiga jenis anggrek Phalaenopsis hasil persilangan dengan konsentrasi EMS terhadap variabel jumlah akar.Anggrek P. 717 X P.Fire Bird memiliki jumlah daun, jumlah akar, jumlah tunas, panjang daun dan panjang akar yang lebih baik dibandingkan jenis lainnya. Konsentrasi EMS secara mandiri pada semua taraf perlakuan tidak dapat meningkatkan jumlah daun, jumlah akar, bobot segar planlet, panjang daun, dan panjang akar.Kata Kunci: Phalaenopsis, Konsentrasi EMS, dan Budidaya In Vitro

Penelitian ini bertujuan untuk mendapatkan media tanam yang mampu memberikan pertumbuhan terbaik terhadap anggrek Phalaenopsis fimbriata. Penelitian ini dilaksanakan di Screen house, Puskopad, Kecamatan Tanjungsari, Kabupaten Sumedang dengan ketinggian tempat 850 meter di atas permukaan laut (Mdpl). Rancangan yang digunakan dalam penelitian ini adalah Rancangan Acak Kelompok (RAK) sederhana dengan 4 ulangan dan 7 taraf, yaitu A = Sphagnum moss, B = Pakis, C = Akar Kadaka, D = Cocofiber, E = Sekam Bakar, F = Cocochip dan G = Hidrogel. Hasil penelitian menunjukan: 1. Berbagai media tanam dapat berpengaruh terhadap pertumbuhan benih anggrek Phalaenopsis fimbriata pada variabel pertumbuhan jumlah daun, panjang daun, lebar daun dan bobot segar.2. Media tanam akar kadaka memberikan pertumbuhan terbaik pada benih anggrek Phalaenopsis fimbriata pada variabel pertumbuhan jumlah daun, panjang daun, lebar daun dan bobot segar.

The experiment was conducted at Faculty of Agriculture UNWIM Tanjungsari - Sumedang, at the altitude of 850 m above sea level (asl) with ground Andisol orders from February to April 2008. The purpose of an experiment to study the effect of the interaction between the concentration of coco water immersion ata dose NPK fertilizer on the growth and yield of melon varieties Action 434. The experimental design used was a randomized block design factorial design consisting of two factors and repeated twice. The first factor is the concentration of water immersion coco (k), which consists of five levels, namely: k0 = 0%, 25% = k1, k2 = 50%, k3 = 75%, and k4 = 100%. The second factor is the dose of NPK fertilizer (d),which consists of four levels, namely: d0 = 0 g plant-1, d1 = 10 g plant-1, d2 = 20 g plant, and d3 = 30 g plant-1. The results showed that there is an interaction effect between the concentration of coco water immersion at a dose of NPK fertilizer to the plants age 20 DAT (days after transplanting) and 30 DAT, as well as the number of leaves at the age of 30 DAT. Coco water immersion at a concentration of 25% gives a better effect on the variable length of the plant, number of leaves, fruit weight per plant, and the quality of the fruit skin. Coco water immersion at a concentration of 50% gives better effect on thevariable sweetness of the fruit flesh. Without dosing NPK fertilizer already gives a better effect on the variable length of the plant, number of leaves, fruit weight per plant, diameter of fruit per plant, and the thickness of the flesh of the fruit. NPK fertilizer dose of 10 g plant-1 give better effect to the variable length of the plant, number of leaves, fruit weight per plant, diameter of fruit per plant, the thickness of the flesh of the fruit, the sweetness of the fruit, and the quality of the fruit skin.

Purpose : Both quantity and quality potato seed production in Indonesia is relatively low, because it is still cultivated conventionally. Aeroponic cultivation can produce seeds in large quantities and quality with environmental control through setting the spacing and temperature regulation of the rooting area (room box). This research was to study the increase in productivity of G0 type potatoes at planting distance and the room temperature of the box. Research Method : The research to study the productivity of G0 type potatoes at different planting distance (j0: 20 cm x 20 cm, j1: 20 cm x 25 cm, and j3: 20 cm x 30 cm), and the room temperature of the box (t0: room temperature (control) and t1: 150C. The experiment was set up as randomized block design and repeated 4 times. Data were analyzed for variance and Duncans' further test. Findings : The results showed no interaction between planting distance and temperature. The planting space of 20 cm x 20 cm gave a significant effect on plant height at the age of 45 DAP. The temperature treatment of 150C has a significant effect on plant height at 15, 30, 45 and 60 days after planting (DAP), a number of leaves at 15 and 45 DAP, and the number of potential tubers. Limitations : Limititations in this research was that harvest time was not optimal because due to the plants inability to continue their lives due to wilting disease and high air temperatures. Value : Information from this research is very important for potato seed farmers in increasing crop productivity by managing temperatures to cool down at night in the rooting area.