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The influence of macronutdents and growth regulators on m vitro shoot proliferation and rooting of an East Spanish population of Myrtus communis L. were studied. Preincubation of field material on a medium without mineral salts prevented the browning from phenolic exudates. For multiplication, nodal segments of 5 mm from in vitro produced shoots were cultured on Murashige and Skoog (MS), Schenk and Hildebrandt (SH) and Heller (H) media (full strength or diluted to 1/2 or 1/4), with 6-benzylaminopurine (BAP) at concentrations 4.4, 13.3 and 22.2 pM or kinetin (K) at concentrations 4.7, 14.0 and 23.2 ~M. The optimum shoot proliferation was on quarter-strength MS medium with 4.4 pM BAP, whereas the maximum number of nodal segments was produced on half-strength MS medium with 4.4 ~VI BAP. Rooting of shoots was obtained by adding 2.5 -24.6 pM indole-3-butyric acid (IBA) and broad range of macronutrients; Lloyd and McCown (WPM) and Crresshoff and Doy (GD) media both full strength or diluted to 1/2 were optimum. No rooting was obtained in the presence of r acid (NAA).