Castration in domestic and companion animals plays an important role in controlling animal population, aggressive male behaviour and producing meat with a consistent quality acceptable to consumers. Surgical castration is the widely used method, but it is more expensive and painful if not well managed. This research focused on the use of 10% iodine and 1% polidocanol foam as intratesticular injections to achieve castration in three adult dogs owned by a client in Lwandeti village in Kakamega County, Kenya. Two of the dogs received 1ml of 1% polidocanol, while one dog received 1ml of 10% iodine in each of their testicles. The testicles were surgically harvested after three months for histological examination. The histological examination revealed that the seminiferous tubules of testes those received 10% Lugol’s iodine had cellular infiltration and fibrin deposition. Those that received 1% polidocanol showed moderate to severe cellular infiltration but less than the iodine group. This study has shown the potential of polidocanol and iodine as chemical methods that can be used instead of surgical castration.