Defense related proteins in the interaction Sporisorium scitamineum-sugarcane. Laboratory experiments were conducted in the National Centre for Animal and Plant Health during 2001-2005. In this paper were determined superoxide dismutase iso-enzyme patterns and transcripts sequenced from PRs on the Sporisorium scitamineum-sugarcane interaction, for this reason were inoculated buds of both varieties Ja60-5 (susceptible) and M31/45 (resistant) with a suspension of pathogen teliospores. Native electrophoresis separation was detected two acidic isozymes, one was induced in high level in resistant varieties and the other at basal constitutive level in both varieties and differential displays sequence protein on resistant cultivar. The cADN-AFLP analysis permited to obtein sequences of PRs homologues to a glucanasas (PR2), quitinasas (PR3) and osmotina (PR5) diferentially induced on resistant variety. These results confi rm that superoxido dismutase enzyme is involved in signal pathway on this interaction; and PRs induction on induced defence response and there are possible use as biochemical and molecular marker on the Sugarcane Breeding Program.