Anemia which is associated with poor maternal and perinatal outcome, is the most common medical disorder and a risk factor in pregnancy causing 20-40% of maternal deaths directly or indirectly through cardiac failure, preeclampsia, antepartum hemorrhage, postpartum hemorrhage and puerperal sepsis. The study was aimed to assess knowledge of pregnant women about iron deficiency anemia at obstetrics and gynecology hospital in Karbala city and to find out the associations between Knowledge Levels and their Socio-Demographic and clinical Data.  An accidental sample (N= 85) of pregnant women who attended the obstetrics and gynecology hospital in Karbala city for receiving antenatal care was included in the study. The questionnaire utilized in data collection include; socio-demographic and clinical data, obstetric and health history and knowledge assessment, to assess the Knowledge of pregnant women regarding to Iron deficiency anemia. The findings indicated that the level of knowledge was moderate among pregnant women. The majority of participants (49.4%) have a High Knowledge and minority of them (24.7%) were of Poor Knowledge and 25.9% had fair Knowledge .The study concludes that the majority of pregnant women in Karbala city have moderate knowledge regarding iron deficiency anemia.