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In this study, emulsion stabilised by soybean soluble polysaccharide (SSPS) and octenyl succinic anhydride modified SSPS (OSA‐SSPS) with high molecular weight (HMW) at concentrations (2%, 3%, 4%, 5%, 6%) was prepared. The droplet size of emulsion stabilised by OSA‐SSPS (HMW) was between 150 and 300 nm with a single distribution peak and increased by 0.1 μm after 4 weeks; that of emulsion stabilised by SSPS was between 1 and 5 μm with two distribution peaks and increased by more than 2 μm after 4 weeks. The viscosity of emulsion with OSA‐SSPS (HMW) was larger than that with SSPS, and the absolute value of zeta potential of microemulsion with SSPS was lower than 43 mV while that of nanoemulsion with OSA‐SSPS (HMW) was higher than 46 mV. The stability of nanoemulsion with OSA‐SSPS (HMW) slightly varied with the increase of storage time and concentration, which provided theoretical guidance for the application of OSA‐SSPS in nanoemulsion.

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