This work involves preparation of a series of 1,2,3-triazole derivatives. In the first step, the reaction of N-acetyl-5H-dibenzo [b,f] carboxamide with different benzaldehyde derivatives to yield chalcone compounds A-D was carried out. In the second step, compounds A-D reacted with 4,4' sulfonylbis(azidobenzene) (G) to produce 1,2,3-triazole derivatives A 1 -D 1 . All the prepared compounds were characterized by Fourier-transform infrared spectroscopy (FTIR) and melting point, some of them were characterized by proton nuclear magnetic resonance ( 1 H-NMR), spectroscopy analysis. Biological activity test was done to evaluate the antibacterial activity of eight synthesized derivative compounds against two multi-drug resistant pathogenic bacteria isolated from patients infected with burn infection; Staphylococcus aureus and Pseudomonas aeruginosa. Three concentrations were selected 50, 100 and 150 mg/mL from each of the synthesized derivative compounds. The derivative compound D 1 with the concentrations of 100 and 150 mg/mL exhibited excellent effect against P. aeruginoa with inhibition zone diameters of 28.10 ± 0.5 and 28 ± 0.05 mm, respectively.