Clostridium chauvoei is one of the deadly micro-organism that causes disease in cattle and sheep. We tested Clostridium chauvoei on different culture media, physio-biochemical agents and antibiotics. The best grwoth of organism was observed on blood and nutrient agar at pH 7.2-7.5, and temperature in between 37-40ºC. No effect of centrifugation was observed. Fourteen different antibiotics were tested against the Clostridium chauvoei. Highly effective antibiotics were chloramphenicol, tetracycline, baquiloprim/sulphadimidine, erythromycin, gentamicin, compound sulphonamides.