Aim:The aim of this study is to compare and to evaluate sealing ability of newly introduced C-point system, cold lateral condensation, and thermoplasticized gutta-percha obturating technique using a dye extraction method.Materials and Methodology:Sixty extracted maxillary central incisors were decoronated below the cementoenamel junction. Working length was established, and biomechanical preparation was done using K3 rotary files with standard irrigation protocol. Teeth were divided into three groups according to the obturation protocol; Group I-Cold lateral condensation, Group II-Thermoplasticized gutta-percha, and Group III-C-Point obturating system. After obturation all samples were subjected to microleakage assessment using dye extraction method. Obtained scores will be statistical analyzed using ANOVA test and post hoc Tukey's test.Results:One-way analysis of variance revealed that there is significant difference among the three groups with P value (0.000 < 0.05). Tukey's HSD post hoc tests for multiple comparisons test shows that the Group II and III perform significantly better than Group I. Group III performs better than Group II with no significant difference.Conclusion:All the obturating technique showed some degree of microleakage. Root canals filled with C-point system showed least microleakage followed by thermoplasticized obturating technique with no significant difference among them. C-point obturation system could be an alternative to the cold lateral condensation technique.

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Aim:The aim of this study was to investigate the correlation between endodontic clinical signs and symptoms and the presence of Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia or their association by Multiplex polymerase chain reaction assay.Materials and Methods:Microbial samples were taken from 30 cases with necrotic pulp tissues in primary infections. DNA was extracted from the samples, which were analyzed for the presence of three endodontic pathogens by using species-specific primers.Results:P. gingivalis, T. denticola, T. forsythia, and Red Complex were present in 11, 17, 4, and 2 canals, respectively. Clinical and statistically significant relationships were found between T. forsythia and mobility and between T. denticola and swelling. (P < 0.05). Presence of other Red complex bacteria shows clinical association with presence of signs and symptoms but no statistically significant relationship.Conclusion:The high prevalence of P. gingivalis, T. denticola, and T. forsythia in the examined samples suggests that these bacteria are related to the etiology of symptomatic periradicular diseases.

Aim: Comparative evaluation of the efficacy of CPP-ACPF and Sodium fluoride with tri-calcium phosphate  on enamel remineralisation using the Diagnodent.

Materials & method: Thirty extracted human premolars were selected and divided into three groups of 10 teeth each: A (artificial saliva), B (CPP-ACPF), C (Sodium fluoride with tri-calcium phosphate -Clinpro 5000). All the samples were assessed using DIAGNOdent at the baseline and after demineralization and remineralisation after 7 days.

Result: Statistical analysis showed that group B(CPP-ACPF) and group C(Clinpro 5000) had significantly higher amount of remineralisation than group A(artificial saliva).

Conclusion: All the three groups showed a statistically significant amount of remineralization. However, Clinpro 5000 showed marginally more amount of remineralisation than CPP-ACPF.