The regulatory mechanisms of pollen development have potential value for applications in agriculture, such as better understanding plant reproductive regularity. Pollen-specific promoters are of vital importance for the ectopic expression of functional genes associated with pollen development in plants. However, there is a limited number of successful applications using pollen-specific promoters in genetic engineering for crop breeding and hybrid generation. Our previous work led to the identification and isolation of the OsSUT3 promoter from rice. In this study, to analyze the effects of different putative regulatory motifs in the OsSUT3 promoter, a series of promoter deletions were fused to a GUS reporter gene and then stably introduced into rice and Arabidopsis. Histochemical GUS analysis of transgenic plants revealed that p385 (from −385 to −1) specifically mediated maximal GUS expression in pollen tissues. The S region (from −385 to −203) was the key region for controlling the pollen-specific expression of a downstream gene. The E1 (−967 to −606), E2 (−202 to −120), and E3 (−119 to −1) regions enhanced ectopic promoter activity to different degrees. Moreover, the p385 promoter could alter the expression pattern of the 35S promoter and improve its activity when they were fused together. In summary, the p385 promoter, a short and high-activity promoter, can function to drive pollen-specific expression of transgenes in monocotyledon and dicotyledon transformation experiments.

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With the changing times, people's demand for a healthy diet is increasing. As a populous country with rapid economic and technological development, China has great potential in the plant meat market. To make a reasonable analysis of the development of Beyond Meat in the Chinese market, this paper will study the current situation of Beyond Meat in the Chinese market through PESTEL analysis, SWOT analysis, and financial analysis, and find out the disadvantages and deficiencies of the company in its growth. We find that Beyond Meat has huge numbers of retail stores and lots of restaurants in the United States, and the number of customers is relatively stable. However, Beyond Meat's financial report shows that the epidemic is affected by increased transportation costs and other factors, which make it suffer great loss. Then the future development direction of Beyond Meat is predicted by discussion, which helps Beyond Meat grow better in China.