Cucumbers (Cucumis sativus L.) originated from the South Asian subcontinent, and most of them are fragile to cold stress. In this study, we evaluated the cold tolerance of 115 cucumber accessions and screened out 10 accessions showing high resistance to cold stress. We measured and compared plant hormone contents between cold-tolerant cucumber CT90R and cold-sensitive cucumber CT57S in cold treatment. Most of the detected plant hormones showed significantly higher content in CT90R. To elucidate the role of plant hormones, we compared the leaf- and root-transcriptomes of CT90R with those of CT57S in cold stress treatment. In leaves, there were 1209 differentially expressed genes (DEGs) between CT90R and CT57S, while there were 703 in roots. These DEGs were not evenly distributed across the chromosomes and there were significant enrichments at particular positions, including qLTT6.2, a known QTL controlling cucumber cold tolerance. The GO and KEGG enrichment analysis showed that there was a significant difference in the pathway of plant hormone transductions between CT90R and CT57S in leaves. In short, genes involved in plant hormone transductions showed lower transcription levels in CT90R. In roots, the most significantly different pathway was phenylpropanoid biosynthesis. CT90R seemed to actively accumulate more monolignols by upregulating cinnamyl-alcohol dehydrogenase (CAD) genes. These results above suggest a new perspective on the regulation mechanism of cold tolerance in cucumbers.
Abstract. To investigate the phenotypic variation of cucumber photosynthesis, net photosynthetic efficiency of cucumbers were measured by LI-6400XT Portable Photosynthesis System. Non-rectangular hyperbolic model was adopted to fit the light response curve. We used 16 cucumber accessions selected from the core collection that consists of over 400 accessions. As a result, cucumbers show a moderate variance in the phenotype of light compensation point (LCP), light saturation point (LSP) and apparent quantum efficiency (AQY), dark respiration rate (R d ), and maximum net photosynthetic rate (A max ). In a detail, the coefficient of variation (CV) of LCP and R d is 48.97% and 48.09%, respectively, which is relatively high. In contrast, the CV values of A max , LSP and AQY are all smaller than 25%. These results help us get better understanding about the phenotypic variation of photosynthesis in cucumber germplasm.
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