Objective: To investigate the effect of Benner Pury on Endothelial progenitor cells (EPCs) proliferation, migration, adhesion, apoptosis and oxidative stress in vitro from patients with hypertension and its time dependence. Methods: The patients who were diagnosed as hypertension grade 1 according to the standard "Guidelines for Prevention and Treatment of Hypertension" were enrolled. Mononuclear cells were isolated by density gradient centrifugation and stained with fluorescence chemical acetylated low density lipoprotein marked with DiI (acLDL-DiI) and fluorescein isothiocyanate (FITC)-lectin. Double staining positive cells were considered as the differentiation of EPCs. The control group included healthy subjects matched with study group in age, gender. EPCs cultivated for 5 days were used for study. Cells harvested at different set times under the stimulation of Benner Pury, and the EPCs proliferation, migration, adhesion ability, apoptosis and oxidative stress indicators were detected respectively. Results: (1) Compared with the control group, peripheral blood EPCs proliferation, migration and adhesion ability were obviously decreased in the hypertension group (p < .01), EPCs apoptosis rate and oxidative stress response were significantly increased (p < .01). (2) Benner Pury significantly increased the EPCs proliferation, migration, adhesion ability and improved apoptosis and oxidative stress reaction in a time-dependent manner. Conclusions: Benner Pury can improve the EPCs proliferation, migration, adhesion, apoptosis and oxidative stress in patients with hypertension in a time-dependent manner.Key Words: Hypertension, Endothelial progenitor cells, Proliferation, Migration, Adhesion, Apoptosis, Oxidative stress Endothelial progenitor cells (EPCs), which are precursor cells of vascular endothelial cells, can be involved in angiogenesis, vasculogenesis and the repair of endothelial injury, and are closely associated with hypertension and target organ damage caused by hypertension.[1] Angiotensin II plays an important role in the occurrence and the development of hypertension. Angiotensin converting enzyme inhibitors (ACEIs) quell the activity of Angiotensin II by inhibiting angiotensin converting enzymes, and then lower blood pressure. This study used EPCs in vitro from patients with hypertension under the intervention of Benner Pury classified as ACEI drugs, on one hand, to observe the changes in EPCs proliferation, migration, adhesion, apoptosis and oxidative stress at different set times, on the other hand, to investigate the effect of ACEI anti-hypertensive drugs on the function of peripheral circulating EPCs in patients with hypertension and further explore whether therapeutical effects of ACEI on patients with hypertension were related to the improve-