Background: Spinal cord injury (SCI) is an unexpected, traumatic event, its consequences often persist for the rest of life. The neurological deficit caused by spinal cord (SC) injury is permanent, and conservative treatment can not completely restore neurological functions in animals. Repeated autogenous adipose-derived stromal vascular fraction (SVF) has been used to deliver MSCs, growth factors such as brain-derived neurotrophic factor, nerve growth factor, and neurotrophin-3 at the site of injury. Methods: In the present study, six dogs presented with SCI, were routinely examined based on standard neurological examination protocol and subjected to the survey radiography. Treatment plans differed as per the presentation of the case. Stable and physically responsive patients were selected for repeated autogenous SVF therapy for rehabilitation. Results: After conservative treatment in SCI, from the second week onwards, when patient supports or able to move affected area in relation to forequarter, the patients were administered repeated autogenous SVF therapy, which restored motor function in the form of intermittent weight-bearing on fetlock and/or posture change. Significance: Spinal cord damage either partial or complete is always an ailment related to neurological deficit of the affected body parts. The repeated administration of autogenous stromal vascular fraction therapy in combination of physiotherapy significantly reverse, the effects of neurological deficit and progressively restore posture and weight bearing on the affected hind quarter in dogs.

The present study was conducted in 12 intact female dogs presented for elective ovariohysterectomy. Animalswere randomly divided into two groups of six each. Group I animals were subjected to conventional ventral midlineovariohysterectomy and in Group II animals, right flank ovariohysterectomy was performed. Clinical efficacyof surgical techniques was evaluated on the basis of clinico-physiological and haemato-biochemical parameters.Surgical duration for performing ovariohysterectomy was comparatively less in right flank approach as comparedto ventral midline. Physiological parameters like rectal temperature showed non-significant variations in all theanimals. Respiratory rate decreased marginally whereas transitory increase in heart rate was observed for short time. Haematological and biochemical observations revealed marginal changes during the period of study in both the groups. The length of surgical incision, duration of procedure and healing were significantly less in Group II as compared to Group I. However, the operative haemorrhage was comparatively more in right flank ovariohysterectomy. The ease of exteriorization and ligation of uterus and ovaries was easy in Group II (right flank approach) as compared to Group I (ventral midline approach). Wound dehiscence was observed in two dogs of Group I whereas Group II animals showed uneventful recovery. Hence, right flank approach is a good alternative over the conventional midventral approach for performing ovariohysterectomy in female dogs

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This study was undertaken for the identification of matrix metalloproteinases (MMPs) in extracts obtained from native, acellular and crosslinked bovine pericardium (in vitro), as well as in the plasma after implantation of these biomaterials in rabbits (in vivo). Native pericardium (NP) expressed a 72 kDa (MMP-2) band; whereas, in acellular pericardium (AP) two bands (10 kDa and 92 kDa) of MMPs were observed of which, 92 kDa band was very faint. AP crosslinked with glutaraldehyde did not show any gelatinase activity and thus reflects the creation of new additional chemical bonds between the collagen molecules which has been effectively removed. Gelatin zymography showed only one major band of 92 kDa in all the implanted and untreated rabbit plasma, but the relative amount of 92 kDa was 1-2 times higher in acellular bovine pericardium implanted rabbits as compared to crosslinked and native groups. In NP group, the 92 kDa band was the dullest among the three groups. This indicated that the level of MMP-9 corresponds to the degree of collagen degradation.

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