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Bi promoted Pd/C catalyst (Bi-Pd/C) was prepared and evaluated for glucose oxidation in this paper. Reaction rate depends on Bi and Pd ratio on activated carbon. Best promotion effect was got when PdCl 2 to Bi (NO) 3 Á5H 2 O weight ratio was 1 to 3 during catalyst preparation. Compared with other noble metal catalyst, the reaction time was reduced and Bi prevented the Pd/C deactivation during this reaction. The reaction cycle for this catalyst was increased a lot compared with other Pd/C catalyst. Through XPS analysis, Bi, Pd was formed on the surface of activated carbon particles. The catalyst reaction cycles for Bi-Pd/C catalyst was exceeded to 20 cycles in spite of the catalyst loss during each cycle. This catalyst can be used for industrial process to oxidize glucose to sodium gluconate.