This study aims to determine the effect of work culture and work commitment on teacher professionalism. This research was conducted at SD Negeri Kayuagung District, Ogan Komering Ilir Regency, Indonesia. The sample of this study was 60 people. The research method uses quantitative. The data collection technique is a questionnaire. The data analysis technique used the normality test, linearity test and t test. The results of the study concluded that there was a significant influence between work culture and work commitment to teacher professionalism.