Tuberculosis (TB) is a contagious infectious disease caused by Mycobacterium tuberculosis. 10 million people suffer from TB Every year. Although TB is a preventable and treatable disease, 1.5 million people die every year due to TB. Alternative treatments continue to be pursued, and treatment with the latest TB drugs that are continuously being encouraged. Black cumin (Nigella sativa) seed contains essential oils with active compounds such as thymohydroquinone, Oleoresins, flavonoids, alkaloids, saponins, tannins, and terpenoids that act as antibacterial drugs. This study aims to determine the sensitivity of N. sativa seed extract in inhibiting the growth of M. tuberculosis strain H37RV and MDR-TB (Multidrug Resistance-TB). This research using Microscopic-Observation and Drug-Susceptibility Assay (MODS) method. Extraction of N. sativa was carried out by the maceration method using 70% methanol as a solvent. The results showed that the M. tuberculosis strain H37RV and MDR-TB were sensitive to N. sativa extract at concentrations of 5 and 10% but resistant to N. sativa extract at concentrations of 1 and 3%.Abstrak: Tuberkulosis (TB) adalah penyakit menular yang disebabkan oleh Bakteri Mycobacterium tuberculosis. Penyakit ini menimbulkan dampak kematian yang cukup mengkhawatirkan. Penyakit tersebut dapat dicegah dan diobati. Salah satu sumber pengobatannya menggunakan biji jintan hitam (Nigella sativa) yang mengandung minyak atsiri dengan senyawa aktif seperti timohidrokuinon, oleoresin, flavonoid, alkaloid, saponin, tanin, dan terpenoid yang berfungsi sebagai obat antibakteri. Penelitian ini bertujuan untuk mengetahui sensitivitas ekstrak biji N. sativa dalam menghambat pertumbuhan M. tuberculosis strain H37RV and MDR-TB (Multidrug-Resistance-TB). Penelitian ini menggunakan metode Microscopic-Observation and Drug-Susceptibility Assay (MODS). Ekstraksi N. sativa dilakukan dengan metode maserasi menggunakan pelarut metanol 70%. Hasil yang diperoleh menunjukkan bahwa bakteri M. tuberculosis strain H37RV dan TB-MDR, kedua strain tsb sensitif terhadap ekstrak N. sativa konsentrasi 5 dan 10%, tetapi resisten terhadap ekstrak N. sativa konsentrasi 1 dan 3%.
: Agricultural waste is composed of carbohydrates which can be be used as a growth medium for bacteria Acetobacter xylinum to produce fermented products in the form of nata. Producing nata from fruit waste such as Cassava peels (Manihot esculenta), Ladyfinger bananas peels (Musa acuminata Colla) and Durian peels (Durio zibethinus) is one of the efforts to reduce environmental pollution. This study aims to compare the quality of nata from Cassava peels (endodermis), Ladyfinger bananas peels (endodermis) and durian peels (endodermis) based on the gel thickness, nata yield and organoleptic test. From the results of the study concluded that Durian peels and Ladyfinger bananas peels could be used as an essential ingredient in making nata. Nata de durio is the best nata product in terms of thickness, nata yield and organoleptic tests. Meanwhile Cassava peels cannot be used as raw material for making nata.Abstrak : Limbah pertanian terdiri dari karbohidrat, yang dapat digunakan sebagai media pertumbuhan bagi bakteri Acetobacter xylinum untuk menghasilkan produk fermentasi dalam bentuk nata. Memproduksi nata dari limbah buah seperti kulit ubi kayu (Manihot esculenta), kulit pisang Ladyfinger (Musa acuminata Colla), dan kulit Durian (Durio zibethinus) adalah salah satu upaya untuk mengurangi pencemaran lingkungan. Penelitian ini bertujuan untuk membandingkan kualitas nata dari kulit ubi kayu (endodermis), kulit pisang emas (endodermis), dan kulit durian (endodermis) berdasarkan ketebalan gel, rendemen nata, dan uji organoleptik. Hasil penelitian menyimpulkan bahwa kulit Durian dan kulit pisang emas dapat digunakan sebagai bahan penting dalam membuat nata. Nata de durio adalah produk nata terbaik dari segi ketebalan, rendeman, dan uji organoleptik, sedangkan kulit ubi kayu tidak dapat digunakan sebagai bahan baku pembuatan nata.
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