A new perspective on the effect of unconditioned indoor (especially storage areas) and outdoor environments on wood acidity is provided in this work. A comparison between the quantity and types of the organic acids formed in the unconditioned indoor environment and different outdoor environments was made. Moreover, the acidity of some wood samples due to different environmental conditions was determined using a pH meter and high-performance liquid chromatography (HPLC). Fourier transform infrared (FTIR) was used to detect the changes in wood components at the molecular level due to environmental conditions. The results suggest that the unconditioned indoor environment was more aggressive than the outdoor environment with respect to wood deterioration. The polluted atmosphere increased the wood acidity and motivated polysaccharide breakdown.