The main task in the dairy farming organization is to obtain calves whose health directly depends on the immuno-biochemical status of their mothers. In our country and abroad, interest in probiotic preparation is increasing to maintain the biochemical and immunological status, to improve digestion, metabolism, as well as to increase the cows' dairy productivity. Probiotic preparations of the Vetom series are widely used in this industry. The aim of the study was to study the effect of "Vetom 1.2" probiotic preparation on the immuno-biochemical status of cows. Experimental studies were carried out in JSC "Uchkhoz "Prigorodnoye" in Barnaul on Russian black pied cows in the autumn-winter period. During the research, we have identified the following: 1. The use of "Vetom 1.2" probiotic preparation has a positive effect on the biochemical parameters' dynamics of cows' blood serum, and therefore, on the immuno-biochemical status of animals; 2. The use of "Vetom 1.2" probiotic contributes to an increase in immunoglobulins' level in cows' colostrum, thereby such colostrum can help to increase the resistance and safety of young animals.