A procedure has been developed to regenerate plants in high frequency from meristems of tomato (Lycopersicon esculentum Mill. cv. Starfire). Shoot apical meristems isolated from 7-day-old seedlings were aseptically cultured on a Murashige and Skoog (MS) medium supplemented with varying concentrations of cytokinins and auxins under defined environmental conditions. Benzyladenine (BA) or zeatin (Z) at various concentrations from 0.1 to 10 µm induced shoot differentiation in high frequency. All levels of BA or Z in conjunction with indoleacetic acid (IAA) also induced shoot differentiation. Whole plant regeneration occurred in media with hormone concentrations as follows: 1) BA and naphthaleneacetic acid (NAA) in the range of 0.1 to 1.0 µm, 2) 10 µm Z in conjunction with 1.0 µm NAA or vice-versa and 3) 10 µm IAA.