Activated water - such water, in which the structural network of hydrogen bonds loosens, water molecules acquire additional degrees of freedom, which facilitates the assimilation of such activated water by the cells of living organisms and accelerates the removal of biological slags. The essence of water activation is the destruction of cluster structures to saturate water with monomolecules. The cluster of activated water contains 5-6 molecules (ordinary water 13-16 molecules). Such water is considered more active in terms of biophysical and biological indicators. Water, activated in any way, has a high fluidity (has a low surface tension) and a dissolving power. Water with micronized clusters has higher reactive properties, better penetrates through biological membranes, is quickly excreted from the body. Effectively replaces and supplements all kinds of cleansing food supplements and physiotherapeutic procedures. Used for complex purification of the body: neutralization and excretion of slags, toxins, carcinogens and radionuclides. The paper considers experimental data on the determination of the total antioxidant activity of water systems saturated with hydrogen using the coulometric method of analysis, with the water having the highest increase in activity up to 20 times. We studied the functional drink “Arkhyz + Antioxidant = Zhivitsa”, all the ingredients that make up it have a natural origin. Saturation of the drink with hydrogen leads to an insignificant increase in the total antioxidant activity (by 16.5% rel.). The presence of active organic compounds in water leads to a decrease in the effect of hydrogen on the total antioxidant activity, probably due to a change in the structure of its aggregates consisting of a different number of molecules.

The article presents the results of the biotechnological study of aquatic environments with molecular hydrogen by antioxidant activity. Molecular hydrogen has selective antioxidant activity against dangerous free radicals. It has anti-inflammatory, anti-apoptotic, anti-allergic effects and stimulates energy metabolism. The problem is urgent nowadays because of the great interest to molecular hydrogen as an antioxidant over the past ten years, but its quantitative metabolic parameters have not been estimated yet. The total antioxidant activity of aquatic systems saturated by hydrogen have been studied using coulometric analysis. It was shown that their antioxidant activity of aquatic systems increase of 8-10 times depending on the operating conditions and time of the "H2 Inhaler" device and 3 times when using a mineral generator active Hydrogen "Aquaspectr Mineral". The effect of molecular hydrogen have been studied by changing the antioxidant status of the human body, which was determined by changing the total antioxidant activity of urine and oral fluid during inhalation of hydrogen and oral administration of hydrogen-saturated water. The synergistic effects has been revealed in determining the total antioxidant activity of the oral fluid in the range from 14.02 to 17.59% rel. and synergism/antagonism of urine in the range from 37.17 to 27.62% rel., compared with control measurements. The total antioxidant activity was determined using electro-generated bromine, the samples were analyzed on an Expert-006 coulometer (Econix-Expert LLC, Russia) according to our certified method.