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Introduction: Cancer is the second leading cause of death all over the world where among all types of cancer breast cancer is said to be the leading cancer followed by lung cancer. The aim of this study is to find the regulation of chloride intracellular channel protein 1 and caspase -3 mRNA expression by hydroethanolic extract of Aegle marmelos fruit human breast cancer cell line-MCF-7. Materials and methods: MCF-7 cells were collected from NCCS Pune, India. It is stored in Dubecos Modified Eagle's Medium. The Aegle marmelos fruit was collected from the herbal department and its extract was prepared. The extract of Aegle marmelosis used in treating MCF-7 cells at different dosages in in vitro.  Isolation of total RNA from MCF-7 cells. The cells will be mixed with total RNA isolation reagent, sonicated and RNA will be isolated as per the standard method. c-DNA conversion and real time polymerase chain reaction. The c-DNA will be synthesized using reverse transcription by commercially available (RT-PCR) kit. Two microlitres of c-DNA will be used for amplification of clic-1 and caspase-3 using gene specific primers by commercially available RT-PCR kit (SyBr kit) and comparative CT method will be used to see the expression of genes. Untreated MCF-7 cells were compared with MCF-7 cells treated with various concentrations of the extract (10, 20 and 40ug). The statistical data’s were collected from the SPSS software version 21. Result: The given extract inhibits the proliferation of MCF-7 cells therefore said to have antiproliferative activity. Different doses of extract were tested (200ug-500ug) out of which 400ug of extract were preferred. Conclusion: The given plant extract has anti proliferative properties and hence can be used as a drug to treat breast cancer.

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<p>Technology has its wide range of applications in every field potentially even on the Mobile technology. One of the technologies which accomplish the blind person in android mobile is Virtual Reality. In spite of virtual reality is engaged to effectuate the operations, the blind person attention is one of the most important statistics. Although mobile devices include operative features available for amaurotic users, the user interface of the greater part of the mobile apps is designed for sighted people. If they carry out any mistakes to use the apps it may lead to a wrong call. So, one may think of a technology that diminishes the anxiety of a blind person for using apps. The proposed system converts the text into audio for giving the directions to the blind person about the gestures inferred. For such conversion a technique called speech synthesizer is used. Assorted innovative tools are used in mobile phones. Blind people need to confide in normal person for creating and updating a contact. Our project incorporates the gestures from the blind people and confirms the gestures through voice. Gesture conversion is accomplished by using haptic technology. An amaurotic person can create a new contact and they can invoke the contact by using this voice confirmation. During calling if there is multiple contact list, caller setting are initialized and ask the preference from the blind people, progress the voice call.</p>

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