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We have purrfied vasoactrve polypetrdes from the urme of normotensrve humans by gel filtratron on Sephadex G150 superfine romc exchange chromatography on DEAE-cellulose and rsoelectnc focusing m polyacrylamrde gels usmg a pharmalyte pH range of 2.5-5.0 The purified polypetrde fraction yrelded four bands by rsoelectrrc focusing with rsoelectrrc pomts at pH 4 15, 4 05, 3 9 and 3 8 On dodecyl sulphate/polyacrylamide gel electrophoresrs (page) two bands appeared, one small band wrth a M, of 29 000 and one broad band rangmg from M,49 000 to M,42 500 The polypetrdes lower the blood pressure of rabbits m a bioassay and cleave D-valyl-L-leucyl-L-argmme-4-mtroanihde with a specrfic actrvrty comparable to that of kalhkrem