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Emetine alkaloid was successively separated for the first time from the alcoholic extract of the four varieties of Hedera helix L. growing in Egypt. The identification of the alkaloid was achieved by applying I. R., U. V., N. M. R. and Mass spectral analysis, as well as, preparation of derivatives. Alkaloids or nitrogenous bases have never been reported in Hedera helix I.. During the course of preliminary chemical screening of the different organs of the four varieties growing in Egypt. viz., Hedera helix var. baltica HORT, H. helix var. hibernica KIRCHN., H. helix var. marginata and H. helix var. erecta SCHULZE, their alcoholic extracts were found to give positive tests for alkaloids and/or. nitrogenous bases (MUNIER and MACHEBOEUF, 1951). It was deemed of interest, therefore, to try the isolation and identification of these compounds. Experimental Preparation of the alcoholic extract. The powdered whole plant material (5 Kg) was exhaustively extracted with 96% alcohol (20 I). The alcoholic extract was concentrated under vacuum at a temperature not exceeding 40°, to a syrupy consistancy (500 ml). This extract was found to give positive alkaloidal tests (MUNIER and MACHEBOEUF, 1951). T. L. C. of the alcoholic extract. By chromatographing the alcoholic extract on T. L. C. plates of Silica gel G (E. Merck), and the plates were developed with the solvent systems recorded in table I, visualised with modified DragendorWs reagent, after drying, it was evident that the alcoholic extract contained four chromatographically separated substances. ' 4 : 1 (v/v) 4. Chloroform-Methanol 9 : 1 (vlv) 5. Chloroform-Methanol-Acetic Acid 90 : 10 : 0.5 (v/v/v

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