Background and objective: Enterococcus species are a common nosocomial pathogen that are gaining resistance to multiple drugs. Thepresent study aims to determine the prevalence of Vancomycin resistant enterococci and susceptibility pattern of urinary isolates ofEnterococci against different antimicrobials and special emphasis on urinary antimicrobial nitrofurantoin and Fosfomycin.Materials and Methods: In the present study, a total of 80 isolates of Enterococci were isolated from urine sample. The antibioticsusceptibility testing was determined for antimicrobials by Kirby-Bauer disc diffusion method. The results were interpreted according toCLSI guidelines, comparative evaluation of vancomycin susceptibility was done by disc diffusion and automated VITEK2 system.Results: 42 isolates were identified as E. faecalis and 38 isolates were identified as E. faecium by VITEK 2. 95% of the Enterococcalstrains were sensitive to Fosfomycin and 65% were sensitive to nitrofurantoin. Among 80 enterococcal isolates, 10 isolates wereidentified as Vancomycin-resistant Enterococci (VRE) by both VITEK2 and disc diffusion. All VRE isolates were found to be sensitiveto Fosfomycin and 50% of VRE isolates showed to be sensitive to nitrofurantoin.Conclusion: Our study showed that Fosfomycin had a good invitro activity for both Enterococci and VRE when compared toNitrofurantoin and other drugs. Vancomycin susceptibility detected by VITEK 2 and by disk diffusion showed good (100%) categoricalagreement.