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(1) Background: It is known that RNA in Na+ salt form has only immunomodulatory activity, and in acid form additionally acquires anti-inflammatory activity and in combination with D-mannitol acquires an even broader antiviral effect. The study aimed to study the ability of adenosinmonophosphat (AMP) and oligoribonucleotides (ORNs) in acid and salt form and in combination with D-mannitol to affect the conformation and fluorescence of interferon (INF) α-2b and to determine the energy parameters of these interactions. (2) Methods: spectroscopy (time pulsed and fluorescence), isothermal nanocalorimetry. (3) Results: AMP and ORNs in acid form and complex with D-mannitol bind more strongly to interferon α-2b than salt analogues. In the interaction of interferon α-2b and acid AMP and especially in complex with D-mannitol, the reaction occurs exothermically and change in conformational mobility INF by increasing the content of disordered regions. When INF α-2b interacts with salt AMP, the reaction occurs endothermically, and probably the salt form increases the conformational stiffness of INF α-2b. The greater efficiency of nonradiative energy transfer from INF α-2b to acid AMP has been shown, due to the closer distance between molecules. (4) Conclusions: AMP in acid form interacts more actively and increases the conformational mobility of INF, at a greater relative distance and with less Gibbs energy compared to the salt form, which probably causes the appearance of additional biological properties of acidic AMP.

Институт молекулярной биологии и генетики НАН Украины 252143, Киев, ул. Академика Заболотного, 150 Разработан способ полунения фермента р-галактозидазы (ЕС 3.2.1.23) в клетках Е. coli с использованием бактериофага. Выход фермента составлял 700 ед. активности в J мл культуральной среды. Накопление фермента в культуральной среде позволило использовать для предвари тельной очистки fi-галактозидазы мембранную технику. Сконцентрированный и частично очи щенный препарат fi-галактозидазы доочищали методом лиганд-аффинной хроматографии. Конеч ный препарат р-галактозидазы характеризовался высокой степенью очистки и нативности. Процессы получения и очистки фермента Р-галактозидазы были выполнены в опытно-промышлен ном варианте.

A simple and efficient method for obtaining nonlysogenic bacteria from lambda-lysogenized strains using phage with an inserted antibiotic-resistant plasmid is described. When the lysogenic culture is infected with antibiotic-resistant phage, single non-lysogenic cells are lysogenized, isolated on selective medium, and cured of phage during incubation at 37 degrees C.