The proximate composition, physicochemical analysis and characterization of gossypium hirsutum seed and its oil was carried out. The parameters that were studied in proximate analysis include (percentage) % moisture, % crude protein, % crude fiber, % ash content, % lipid. The total % carbohydrate content of the seed was 30.49+0.4 and the estimated % energy value was 435.58. The chemical parameters analyzed on the oil include iodine value, saponification value, free fatty acid (FFA) value and % volatile matter. In the characterization of fatty acid present in the oil with GC-MS Spectroscopy system, the major unsaturated fatty acid values were 14.53% for oleic acid and 55.38% for linoleic acid while the palmitic acid and stearic acid values which were saturated acid are 27.39% and 2.23% respectively. The percentage values of the rest of the fatty acid present in the oil were very low. The parameters determined were within the international and Nigerian industrial standard for vegetable oil.