Vancomycin resistance and high level aminoglycoside resistance in Enterococci has limited its treatment, as Enterococci are susceptible to only narrow spectrum of antibiotics. VRE is known to cause hospital acquired infections. This study was aimed to determine prevalence of vancomycin resistance and HLG resistance among Enterococci in a Pediatric hospital. 310 Enterococcus spp was isolated between Jan 2018-May 2020 from pediatric patients from various samples. Among them 206 (66.5%) isolates were Enterococcus faecalis, 104 (33.5%) isolates were Enterococcus spp including Enterococcus faecium. Most of the isolates were from urine (68%). High Level Gentamicin (HLG) resistance was seen in 69% of the isolates. Vancomycin resistance (VRE) was seen in 29 (9.4%) isolates. HLG resistance among VRE was 79% (23/29), all of them were sensitive to Linezolid. 17 of the VRE isolates were from urine sample and 15 (88%) of these isolates were sensitive to Nitrofurantoin. 90% (26/29) of the VRE isolates were isolated from nosocomial infections. 58% of VRE isolates were Enterococcus faecalis.