The anti fungal effects of some plants extracts namely tobacco leaf, keora seed, keora, mahogoni, gaint indian milky weed, garlic and ginger at different concentrations (30%, 40%, 50%, 60% and 70%) on the growth and development of C. gloeosporioides, causal agent of anthracnose of mango were evaluated. Radial growth of C. gloeosporioides was recorded. The growth inhibition increase with the increase of concentration of all the plant extracts. Highest mycelial growth inhibition (74.35%) was observed in case of garlic extracts at 70% concentration. Garlic extract at 50% and 60% concentration were also effective than other treatments.
The experiment was conducted to establish an efficient and reproducible protocol for the plant regeneration and genetic transformation in white Jute (Corchorus capsularis L.). The regeneration and transformation processes depend on optimum growth conditions, suitable explants and varieties. An attempt was made for Agrobacterium mediated genetic transformation in white jute varieties using gene construct conferring both salt and drought tolerance (CIPK and Gly-1) along with the marker genes. Interestingly the two varieties (CVL-1 and Tricap-1) showed the response of both callus induction and plant regeneration on a single formulation i.e. MS medium supplemented with 2.0 mg/l BAP and 0.5 mg/l IAA. Explants were dipped to liquid culture of bacteria for one minute and then transferred to co-cultivation media for 24 hours. Shoot regeneration from Agrobacterium infected cotyledon was found highest in variety CVL-1 (43%) than Tri cap (38%). After co-cultivation and selection histochemical GUS assay was performed in different varieties (vars. Tricap-1, CVE-3 & CVL-1). In the transformed explants, GUS reporter gene was expressed showing blue colour in the explants tissues. Among the varieties CVE-3 showed the highest expression blue colour in the explants tissues. Those transgenic plants are transferred to salt medium and soil for evaluation. DOI: http://dx.doi.org/10.3329/bjar.v37i1.11180 Bangladesh J. Agril. Res. 37(1): 97-107, March 2012
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