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Although it has been shown that tracheal epitheial cells in culture synthesize and secrete phospholipids, no direct cvidence for in situ phospholipid storage in human respiratory secretory epitheial cells has been demonstrated. We used a high-resolution cytochemical enzyme-gold technique to identify and precisely localize phospholipids in human submucosal gland secretory cells. In addition, lysozyme, a specific serous cell marker, was identified using the biotinstreptavidin gold technique with lysozyme antiserum. This double labeling of phospholipids and lysozyme was performed using gold partides of diameters 15 nm and 5 nm, Preparation ofPhospholipase A2-Gold Complex. The phospholipase A2-gold (PLA2) technique depends on the ability of the bee venom PLA2

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