Abstract. Bone is highly organized material from the gross macroscopic to the molecular level. Bone is natural composite material, which by weight contains about 60% mineral, 30% matrix and 10% water. Spectro chemical method is described for the determination of major, minor and trace elements in animal bone. In the present study we analyse bovine metacarpus bone sample by spectro chemical analysis using Jerrel ash plane grating spectrograph and we find out major and minor constituents present in it. Analytical methods are described for the determination of major, minor, and trace elements in bone. V, Ca, Mo, Pb, Fe, Zn, Cu, Mg, Al, P are identified in the bovine metacarpus bone as major trace elements and the 16 elements are identified as minor trace elements i.e., Os, Sc, Y, K, Mn, La, Cd, Pd, Ru, Na, Sn, Pt, Bi, As, Si and Co. It is interesting to note that very low amount of trace elements have been shown to be essential to the growth of animal bones.