We have developed a new method for identifying specific single- or double-stranded DNA sequences called nicking endonuclease signal amplification (NESA). A probe and target DNA anneal to create a restriction site that is recognized by a strand-specific endonuclease that cleaves the probe into two pieces leaving the target DNA intact. The target DNA can then act as a template for fresh probe and the process of hybridization, cleavage and dissociation repeats. Laser-induced fluorescence coupled with capillary electrophoresis was used to measure the probe cleavage products. The reaction is rapid; full cleavage of probe occurs within one minute under ideal conditions. The reaction is specific since it requires complete complementarity between the oligonucleotide and the template at the restriction site and sufficient complementarity overall to allow hybridization. We show that both Bacillus subtilis and B. anthracis genomic DNA can be detected and specifically differentiated from DNA of other Bacillus species. When combined with multiple displacement amplification, detection of a single copy target from less than 30 cfu is possible. This method should be applicable whenever there is a requirement to detect a specific DNA sequence. Other applications include SNP analysis and genotyping. The reaction is inherently simple to multiplex and is amenable to automation.

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Mars Polar Science is a subfield of Mars science that encompasses all studies of the cryosphere of Mars and its interaction with the Martian environment. Every 4 yr, the community of scientists dedicated to this subfield meets to discuss new findings and debate open issues in the International Conference on Mars Polar Science and Exploration (ICMPSE). This paper summarizes the proceedings of the seventh ICMPSE and the progress made since the sixth edition. We highlight the most important advances and present the most salient open questions in the field today, as discussed and agreed upon by the participants of the conference. We also feature agreed-upon suggestions for future methods, measurements, instruments, and missions that would be essential to answering the main open questions presented. This work is thus an overview of the current status of Mars Polar Science and is intended to serve as a road map for the direction of the field during the next 4 yr and beyond, helping to shape its contribution within the larger context of planetary science and exploration.