An in-depth study of the biology of tumor growth will help to identify factors that allow us to understand the pathogenetic mechanisms of the development of ovarian cancer metastasis and progression, as well as to become a theoretical basis for developing new approaches to the treatment of this disease. The aim of this study was to determine immunohistochemical and endothelial criteria for ovarian cancer. The postoperative samples of ovarian tumor tissues were divided into 3 groups: comparison group - ovarian cancer; main group - borderline ovarian tumor; benign ovarian tumors. The study was conducted according to the FIGO 2009 classification. The International Histological Classification of WHO 2013 Female Genital Tumors was used for morphological characteristics. Level of growth factors - sVEGF-A was performed by ELISA using standard test systems (BenderMedSystem, Austria). IGC material studies were performed on serial paraffin sections using a standard method with murine monoclonal antibodies to p53 (clone D0-7. Dilution 1: 100. "Dako"). Ventana Medical Systems, Inc. was used as the detection system. Positive and negative control reactions were performed. The label index (MI) was used to evaluate p53 nuclear expression. WCIF ImageJ and Aperio Image Scope were used to estimate the number and degree of cell staining. Statistical analysis of the obtained data was performed using Statistica 6.0. The results of morphological studies of ovarian cancer showed that in our study patients with serous cancer predominated - 78.5%. The second most frequently diagnosed cancer was undifferentiated. In the second stage of our study, we conducted a comparative analysis of the concentration of p53 in the serum and tissue of patients in the study groups, which showed the existence of significant differences. In patients of the POY and DOY groups, both total and local p53 protein activity were significantly higher than in the comparison group, p <0.05. There was a positive correlation between p53 protein activity in serum and ovarian tissue. Serum VEGF A scores were statistically significantly correlated with the disease stage: Spearman rank correlation coefficient rho = 0.30; 95% CI = 0.02 - 0.536, p <0.05. There were no correlations with patients' age, histological subtype, and degree of tumor differentiation. Considering the results of our study, we can conclude that the criterion-important indicators of QA are serum levels of p53 and the index of serum VEGF A, which is confirmed by the results of ROC analysis p = 0.0026, and indicates a good informativeness of the method.

Objective: to study the effect of nabumetone in oncogynecologic patients in the postoperative period. Materials and methods. There were examined 98 patients of 40-78 years old, who were divided into two groups: the main group - 58 patients, of which 37 - patients with endometrial cancer I-III and 21 - with ovarian cancer of II-IV stage (FIGO); The comparison group included 40 patients (17 and 23 patients respectively). All patients underwent surgical treatment. In the postoperative period, were used NSAIDs - nabumeton. Before the operation and on the 10th day of the postoperative period, the following parameters were studied: a clinical blood test, TNF-a, ceruloplasmin, diene conjugates (DC), malonic dialdehyde (MDA), antioxidant activity (AOA). The content of TNF-a, in the blood serum was determined by radioimmunological, and DC, MDA, AOA by biochemical method. Results. Established: the content of the leukocyte formula before the start of treatment in the compared groups did not reveal any significant differences. After surgical intervention, both groups showed an increase in the absolute values of all leukocyte fractions. The level of ceruloplasmin in the patients of the comparison group increased significantly in the postoperative period, and in the main group the parameters of ceruloplasmin did not differ significantly. The level of TNF-a in the pre- and postoperative period in the compared groups did not differ. On the 10th day of the postoperative period, it significantly increased in the comparison group, and when nabumetone was used during the postoperative period, the level of TNF-a did not increase. The initial indicators characterizing the processes of LPO and AOA in both groups did not differ significantly. Assessing the dynamics of indicators before and after the operation, it was noted that in patients in the blood, the level of DC in both the baseline and in the comparison group increased. The use of nabumethon led to a slight increase in DC. The maintenance MDA in the postoperative period has increased in both groups, however it is unreliable in comparison with parameters before surgical intervention. In the comparison group, after the treatment, the antioxidant defense was reliably reduced. In the main group, the use of nabumetone contributed to a smaller decrease in AOA. The use of nabumeton allowed abandoning of narcotic analgesics in earlier periods of the postoperative period. Conclusion. Use of nabumeton in the postoperative period allows to reduce the pain syndrome, the number of inflammatory complications; contributes to the normalization of LPO processes, inhibits the inhibition of the antioxidant system, which contributes to the reduction of endogenous intoxication and improves the results of treatment of oncogynecologic patients. Key words: nabumeton, oncogynecologic patients, anti-inflammatory action, tumor necrosis factor, ceruloplasmin, diene conjugates, malanialdehyde, antioxidant activity.

To estimate the MSI frequency in colonic mucosal lining and blood serum in patients with endometrial cancer (EC). Methods and results. In 342 patients aged 30-80 with stage I-IV endometrial cancer occurrence of microsatellite instability (MSI) in tumour tissue and blood serum was researched by means of the polymerase chain reaction method. It was established that without regard to carcinoma phenotype MSI this genetic phenomenon is determined signifi cantly more often in blood serum (10.8% of cases) than in colonic mucosal lining (6.1 % of cases). A similar pattern was observed in cases with tumour phenotype MSI+ (MSI frequency in colonic mucosal lining made 12.2%, whereas in blood serum -29.6%, p <0.01).Conclusion. In cases with tumour phenotype MSI-the genome microsatellite instability was observed more often in colonic mucosal lining (3.1%) than in blood serum (1.3%). MSI frequency in colon tissue was higher in EC patients with phenotypic characteristics and risk factors typical for hormone-dependent forms.

Summary. The aim of the study was to study the effect of clinical and morphological factors on diagnosis and onset of disease recurrence. Materials and methods. To achieve this goal, we formed a sample of postoperative samples of ovarian tumor tissue, which were divided into 3 groups: comparison group — ovarian cancer (T1-3N0M0, T1-3N0M1) (РЯ n = 261) main group 1 — borderline ovarian tumor (T1-3N0M0) (singing n = 100); the main group 2 — benign tumors of the ovaries (DOYA n = 40). The age of patients ranged from 23 to 62 years. Of these, 50 % were women of childbearing age — from 23 to 36 years (n = 26). The comparison group included 46 patients diagnosed with ovarian cancer aged 35 to 78 years. Among them, the age group from 30 to 40 years old was 14 % (n = 6). Most of the observations — 56 % (n = 24) — occurred in patients aged 40-60 years. Women over 60 made up 30 % (n = 14). Results and discussion. Ovarian cancer in the structure of the female genital organs malignancy is characterized by an ambiguous forecast and the highest mortality rate. The leading factors determining this phenomenon are the features of tumor metastasis. Metastasis of ovarian cancer occurs at the early stages of the disease and runs a variety of ways: contact, intraperitoneal, haemacirculatory and through the lymphatic system. Such features are determined by the topography of metastatic disease, anatomy, blood supply and lymphatic channel of the female reproductive gland. The greater omentum plays the barrier role in the development of the pathological process of the abdomen. At present poor prognosis and poor treatment outcomes require reviewing approaches to the surgical treatment of ovarian cancer. In this connection defining the features of ovarian cancer metastasis provides an opportunity to find new ways to disable malignant cells distribution. Conclusions. A key role in the treatment of ovarian cancer has surgery, chemotherapy, and targeted therapy. Adequate staging is important in choosing treatment tactics and assessing the prevalence of the disease. The importance of genetic and molecular studies is growing, according to the results of which it is possible to predict an increased risk of developing OC, to individualize and adjust treatment regimens.