Indonesia is one of the largest banana producing countries in the world. In 2019, national banana production was 7,280,658 tons, leaving 4 tons of banana stems for every tonne of bananas harvested. Banana stem fiber has high strength, light weight, high water absorption, fire resistance properties and biodegradable. It has high cellulose contain of 46.3%. Therefore, banana stem fiber is potential for a source of microcrystalline cellulose (MCC). The MCC was successfully isolated 34.9% yield. Edible film was fabricated by solvent casting method. Chitosan was added for antibacterial properties of fabricated edible film. The optimum formulation for chitosan and MCC mass were examined to obtain edible film with good water absorption and biodegradability.