Key words: Vitex negundo, Medicinal plant, Clonal propagationVitex negundo L., a perennial shrub belonging to the family Verbenaceae, is an important medicinal plant. It grows abundantly in St. Martin's Island and commonly known as Nishinda. Generally leaf is used for medicinal purpose but root, flower and fruit also have the medicinal values (Hasan 1982). Leaf of the plant contains essential oil, an alkaloid, nishindin. Stem and bark contain flavonoid glycosides. Leaves of nishinda very effectively reduce the inflammatory swellings of joints in rheumatic attacks. Juice of fresh leaf removes fetid discharges and worms from ulcers. Flower oil is applied to sinuses and scrofulous sores. Root juice is tonic, expectorant and diuretic (Ghani 1998).Tissue culture has greatly enhanced the scope and potentiality of mass propagation by exploiting the regenerative behavior in a wide range of selected horticultural and agricultural plants including the medicinal ones , Thiruvengadam and Jayabalan 2000, Islam et al. 2001and Jawahar et al. 2008. Keeping in mind the medicinal importance of all the parts of this plant, an attempt has been made to formulate its regeneration system through in vitro culture.Plant materials (twigs) of field-grown plants were collected from Kolakupabandura village under Nababganj thana of Dhaka district. Sample was washed thoroughly under running tap water and then with distilled water. Shoot apex of the twigs were then excised and surface sterilized with 0.1% HgCl2 solution for about 7 minutes followed by three to five washes with sterilized distilled water to remove the traces of HgCl2 solution. Shoot tip explants were then cultured in MS supplemented with different concentrations (0.5, 1.0, 1.5 and 2.0 mg/l) of BA, 2iP and Kn singly or in combination with fixed concentration (0.1 mg/l) of NAA. For root induction, excised shoots of in vitro regenerated plants were cultured on half strength of MS supplemented with various concentrations of IBA ranging from 0.5 -1.5 mg/l. In all the cases pH of the media

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