Rambai (Baccaurea motleyana) is one of the underutilized fruits native to Indonesia. Rambai has high antioxidant activities containing phenolic, flavonoid, and anthocyanin compounds, secondary metabolite compounds derived from sugar metabolism. The sugar metabolism involved several related genes. This research aimed to characterize genes putatively involved in sugar metabolism in Rambai. Six sugar gene families were identified from 37 077 contigs of the assembled-transcriptome database against to UniProt database using the BLASTX program. The six sugar-related genes were characterized involved nine contigs of sucrose-phosphate synthase (SPS), three contigs of sucrose-phosphatase (SPP), 14 contigs of sucrose synthase (SUS), 19 contigs of alkaline/neutral invertase (INV), one contig of cytosolic invertase (CINV) and five contigs of beta-fructofuranosidase (CWINV). This research aims to give a comprehensive study of the sugar metabolism mechanism in B. motleyana. The data also revealed the genes that encoded the enzymes that were putatively involved in sugar metabolism.