K (Kell) is one of the most immunogenic of the red blood cell (RBC) antigens. In order to select K− RBC units, we developed K phenotyping on the Olympus PK-7200 equipment to save labor, time, and costs. The Olympus PK-7200 is fully automated equipment used primarily for blood typing and syphilis screening. We tested 3,587 blood donor samples in EDTA using a commercial anti-K serum diluted in HP Hemagen Power Solution R (1:40). The equipment was set to prepare a 1.7% RBC suspension in bromelain and to dispense 25μL of the mixture (diluted serum and HP Hemagen Power Solution R ) in terraced microplates. After mixing, the microplates were incubated for 1 hour at 30°C. Reading was performed by a C.C.D. camera and the results were automatically transferred to the mainframe computer. We found 185 K+ blood samples and 3,402 K− samples. Four samples, K+ by the PK-7200, were confirmed as K− by tube test. The use of bromelain with the PK-7200 may have caused the falsely positive tests. The Olympus PK-7200, used for K phenotyping, saves labor time and costs. It also reduces handling and thus promotes less contamination risk for laboratory personnel. Immunohematology 1998;14:22-25.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
customersupport@researchsolutions.com
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
Copyright © 2025 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.