Introduction: Cucumis sativus fruits are claimed for their antiurolithiatic activity in traditional system of medicine. Materials and Methods: Present study was undertaken to evaluate lithotryptic effect using hydro-alcoholic extract of Cucumis sativus (HCS). Ethylene glycol (0.75% v/v) was used to induce calculi in Wistar albino rats. Assessed various parameters like, Biochemical, Histopathological and routine urine analysis. Results: Treatment with preventive and curative doses of HCS was found to exert dose dependent antiurolithiatic action. Increased urine volume in HCS treated groups as compared to diseased group was indicative of diuretic property. Elevated calcium, phosphate and oxalate levels in diseased group animal were found to be decreased in animals treated with HCS. Increased levels of serum creatinine, BUN and uric acid were considerably brought down towards normal values in proportion to HCS doses administered. Histopathology of kidney reveals severe renal damage due toperformed crystals. Animals treated with HCS showed remarkable recovery, suggestive of prevention of nucleation and aggregation of stone forming components. Conclusion: It can be concluded that, results are supportive to claims made. The exact mechanism of antiurolithiatic effect may further needs to be studied.

Objective: To develop chemical fingerprint profile of secondary metabolites of therapeutically important plant Elephantopus scaber L. using HPTLC technique. Method: Preliminary phytochemical investigation was performed for the detection of secondary metabolites. HPTLC fingerprint profile of hydro-alcoholic extract of E. scaber was developed for alkaloids, flavonoids and saponins. Toluene: Ethyl acetate: Diethyl amine It can be concluded that the HPTLC fingerprint analysis is useful in exact identification of plant material. Also, it serves as a significant tool in differentiating adulterants and biomarker in herbal drug industry.

The present study is aimed at investigating the lithotriptic activity of Nerium oleander L. by inducing experimental urolithiasis in Wistar rats. The screening of lithotriptic effect of hydro-alcoholic extract of Nerium oleander L. 100,200 and 400 mg/kg) was evaluated using ethylene glycol induced urolithiasis in rats. Animals treated with plant extracts (curative and preventive doses) showed promising lithotriptic effects. Particularly low dose (Gr. VI) was more potent compared with medium and high dose of hydroalcoholic extract. Increased urine volume and progressive weight gain was suggestive of diuretic property and protective effect against lithogenesis, respectively, in the animals treated with hydro-alcoholic extract. Increased concentrations of stone-forming components such as calcium, phosphate and oxalate in diseased group were reduced in the animals treated with extracts. Serum creatinine, BUN, and uric acid levels were elevated in calculi induced animals which were close to normal values in animals treated with extract. Severe renal damage was observed in kidney histopathology due to crystal aggregation and deposition. Remarkable recovery with animals treated with extract was confirming about inhibition of process of lithogenesis. It can be concluded that the results are supportive to claims made. The exact mechanism of lithotriptic effect further needs to be studied.