The discovery of DNA plays vital role in Human identification is one of the major fields of study and research in forensic science. This discovery was the basis for the development of techniques that allow characterizing each person's individuality based on the DNA sequence. Individual identification of ancient human remains the most fundamental requisites for studies of paleo-population genetics, including kinship among ancient people, intra-and inter population structures in ancient times, and the origin of human populations. However, knowledge of these subjects has been based mainly on circumstantial archaeological evidence for kinship and intra population structure and on genetic studies of modern human populations. Tooth and several biological materials such as bone tissue, hair bulb, saliva, blood and other body tissues may be employed for isolation of DNA and accomplishment of laboratory tests for human identification. Here we describe individual identification of ancient humans by using short-nucleotide tandem repeats and mitochondrial DNA (mtDNA) extracted from a tooth as genetic markers. The application of this approach to kinship analysis shows clearly the presence or absence of kinship among the ancient remains examined.