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Aeromonas sp. DYU-Too 7 was screened to produce chitinases. The enzymes had an optimal reaction temperature of 50C and an optimal pH of 7.0. The best concentration of chitin in the fermentation process is 4%. Higher concentrations of chitin inhibited microbial growth and influenced the production of reducing sugars, the enzymatic activity and the protein content in the culture. N -acetylglucosamine, the main product in the hydrolysate, was 0.7 g/L at 36 h of cultivation. The concentration of N -acetylchitobiose was 0.16 g/L for the regular chitin broth and 0.28 g/L for the phosphorus-limiting chitin broth, with the highest concentration of N -acetylchitohexaose at about 0.062 g/L in both media. Experimental results showed that cultivation under a phosphorus-limiting condition substantially reduced the production of biomass, chitinases as well as N -acetylchitooligosaccharides.