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SUMMARYPurified Semliki Forest virus was analysed by analytical sedimentation, rate zonal sedimentation, isopycnic sedimentation, polyaerylamide gel electrophoresis and electron microscopy. It was found that the spherical virus particles had a sedimentation coefficient of 241 S (249 S determined by rate zonal sedimentation), and a buoyant density in sucrose of I.I 90 g/ml; one protein and one glycoprotein were present in the virus; after fixation and negative staining with phosphotungstic acid the diameter of the particles was measured to be 50 to 56 nm.

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