The paper presents the results of structure and texture formation study in Ti-6Al-4V alloy samples, obtained by Direct Metal Deposition technology of additive manufacturing. The effect of annealing on the formation of the structure and texture in 3D-samples was investigated. It is shown that the samples have a two-phase structure of “basket weaving” in the initial state and after annealing. It is established that the samples have significant anisotropy of properties in two mutually perpendicular directions that could be explained by the presence of the crystallographic texture.