No abstract

The oxidation o f nicotinamide-adenine dinucleotide phosphate (reduced form, NADPH) at glassy carbon electrodes modified with different enzymes has been studied. It was found that NADPH yielded a well-defined anodic peak about + 0.70 V (vs. Ag/AgCI) at glassy carbon electrodes coated with three different enzymes, glutathione reductase, glutathione peroxidase, and glucose oxidase, in 1.4 M potassium phosphate supporting electrolyte. It was confirmed that the enzymes immobilized at the working electrode surface reduced the overpotential for the oxidation of NADPH. This method provides a way to study the mechanism of some enzyniatic reactions.