The aim ofthis study was to investigate the effect of reproductive hormones in seminal plasma on the quality of the spermatozoa in men seeking infertility treatment. Of the 383 volunteers recruited, 123 had normal sperm parameters and 260 had either a single or a combination of sperm abno rmalities as per WHO criteria . Follicle Stimulating Hormone (FSH), Luteinizing Hormone (LH) Prolactin (PRL), Testosterone (T) and Estradiol (E2) levels in serum and semen of these men were determ in ed using an immunometri c chemi luminisance assay. Of the serum hormones, FSH, LH were significantly higher (p