The nanoparticles of both copper oxide (CuO NPs) and selenium (Se NPs) were synthesized by a laser ablation method that is fast and safe in biological applications. It was Characterization by atomic force microscopy (AFM), Xray diffraction (XRD), field emission scanning electron microscopy (FESEM) and atomic force microscopy (AFM), which showed that the size of the nanoparticles was 20-23 nm for Se NPs and 19-23 nm for CuO NPs. The effectiveness of these nanoparticles was examined on vitamin D levels in the group of women with primary infertility (PP) and the group of women with secondary infertility (SP). The results showed a highly significant activation effect of the copper oxide nanoparticles, with an activation rate equal to 13% for the primary sterility group (P = 0.003) and 23% for the secondary sterility group (P = 0.00

The nanoparticles of both copper oxide (CuO NPs) and selenium (Se NPs) were synthesized by a laser ablation method that is fast and safe in biological applications. It was Characterization by atomic force microscopy (AFM), Xray diffraction (XRD), field emission scanning electron microscopy (FESEM) and atomic force microscopy (AFM), which showed that the size of the nanoparticles was 20-23 nm for Se NPs and 19-23 nm for CuO NPs. The effectiveness of these nanoparticles was examined on vitamin D levels in the group of women with primary infertility (PP) and the group of women with secondary infertility (SP). The results showed a highly significant activation effect of the copper oxide nanoparticles, with an activation rate equal to 13% for the primary sterility group (P = 0.003) and 23% for the secondary sterility group (P = 0.00)