This study assessed the effect of training program on teachers' knowledge of and attitude toward reproductive health education/ sexuality education (RHE/SE) in five randomly selected rural schools in rural area of Wardha district, Maharashtra, India. All the 40 teachers in the selected were recruited for the study. They (40 teachers) were all given training in RHE/SE for 1 month. Their knowledge and attitude toward RHE/SE were assessed pre-and post-training program. The results show a significant increase in percentage of those who had good knowledge in general areas of RHE/SE at post-training assessment compared with pretraining assessment [from 14.3% to 53.6%, p = 0.0001). Also, pre-post attitudinal disposition assessments show that there was an increase in percentage of those who were favorably disposed to the teaching of RHE/SE. The study suggests that RHE/SE should be included and made compulsory in all training program for all teachers in india.